عثمان راشد أحمد الزهراني

 


      
 
الاسم الاول: 
عثمان
اسم العائلة: 
الزهراني
الدرجة العلمية: 
دكتوراة
مجال الدراسة: 
العلوم والتقنية
المؤسسة التعليمية: 
Bangor University

مجال التميز

تميز دراسي وبحثي

 

 

البحوث المنشورة

 

البحث (1):

 

عنوان البحث:

Translin and Trax differentially regulate telomere-associated transcript homeostasis

رابط إلى البحث:

Click here

تاريخ النشر:

10/05/2016

 

 

 

 

 

موجز عن البحث:

 

Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.

 

 

المؤتمرات العلمية:

 

 

 

المؤتمر (1):

 

عنوان المؤتمر:

8th  International Fission Yeast Meeting

تاريخ الإنعقاد:

21/06/2015

مكان الإنعقاد:

Kobe, Japan

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Analysis a novel telomere-associated function of TraX (tfx1+)

 

 

 

 

 

 

 

 

 

 

ملخص المشاركة:

 

Translin and Trax are conserved proteins (fission yeast to human) that have a close functional relationship with one another. They have the capacity to bind and process nucleic acids, and have been functionally implicated in distinct RNA processing pathways, including tRNA precursor processing and human neuronal mRNA transport. In Drosophila melanogaster and human cells they make up the C3PO complex which is a responsible for enhancing the removal of the passenger during RNAi mediated mRNA regulation.

Deletion of both tsn1 (Translin) and tfx1 (TraX) genes in Schizosaccharomyces pombe results in no measurable phenotype (Saccharomyces cerevisiae does not have tsn1/tfx1 orthologues). Given the link to RNAi regulation in D. melanogaster and humans we made a series of double mutants of tsn1 and tfx1 and RNAi genes. Analyses of these strains ultimately lead us to determine that tfx1 mutants were defective in controlling silencing of naturally occurring sub-telomeric genes, suggesting that TraX functions in the regulation of telomere dynamics. Extending this leads us to propose that telomere functional stability is maintained via interplay between Translin/TraX and Ago1. Moreover, these findings point to the possibility that there is a functional ‘trade off’ between telomere maintenance and efficiency of mitotic chromosome segregation.

المرفقالحجم
Japan Certificate of Participation.pdf‏139.88 ك.بايت