هديل محمد علي الاهدل

 


      
 
الاسم الاول: 
هديل
اسم العائلة: 
الاهدل
الدرجة العلمية: 
دكتوراة
مجال الدراسة: 
الطب والخدمات الصحية
المؤسسة التعليمية: 
Bristol University

 

 

مجال التميز

تميز دراسي وبحثي 

 

 

البحوث المنشورة

 

البحث (1):

 

عنوان البحث:

Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma

رابط إلى البحث:

Click Here  

تاريخ النشر:

24 November 2017

موجز عن البحث:

Following peripheral axon injury, dysregulation of non-coding microRNAs (miRs) occurs in dorsal root ganglia (DRG) sensory neurons. Here we show that DRG neuron cell bodies release extracellular vesicles, including exosomes containing miRs, upon activity. We demonstrate that miR-21-5p is released in the exosomal fraction of cultured DRG following capsaicin activation of TRPV1 receptors. Pure sensory neuron-derived exosomes released by capsaicin are readily phagocytosed by macrophages in which an increase in miR-21-5p expression promotes a pro-inflammatory phenotype. After nerve injury in mice, miR-21-5p is upregulated in DRG neurons and both intrathecal delivery of a miR-21-5p antagomir and conditional deletion of miR-21 in sensory neurons reduce neuropathic hypersensitivity as well as the extent of inflammatory macrophage recruitment in the DRG. We suggest that upregulation and release of miR-21 contribute to sensory neuron–macrophage communication after damage to the peripheral nerve. 

 

 

المؤتمرات العلمية:

 

المؤتمر (1):

 

عنوان المؤتمر:

Southwest UK RNA meeting 

تاريخ الإنعقاد:

  02/06/2015 

مكان الإنعقاد:

UK

طبيعة المشاركة:

Oral presentation

عنوان المشاركة:

Investigating the role of micoRNA dependent genes in neural functions 

ملخص المشاركة:

Neurogenesis in the embryonic and adult brain has critical roles in neural development and maintenance of brain function. As Adult neural stem cells (NSCs) continuously generated in two regions, the subgranular zone (SGZ) and the subventricular zone (SVZ), that  have electrophysiological properties, connect with other cells and integrate into the existing neuronal circuitry. Here we wish to investigate the effects of microRNAs on adult neurogenesis. MicroRNAs (miRNAs) are a class of small non-coding RNAs that act as post-transcriptional regulators. They are transcribed as precursor molecules that are subsequently processed into active 21 nucleotide mature miRNA that. miR-21 is a miRNA that has been reported as an oncogene that can increase cell proliferation and reduce apoptotic cell death. However, the role of mir-21 in the adult brain development is still unclear.

Using transgenic mice that either overexpress miR-21 or have loss-of-function in miR-21, we demonstrate that miR-21 over expression led to increased numbers of the DG new-born neurons (DCX+ cells) and mature neurons (NeuN+ cells) in the adult brain in vivo, while knockout of miR-21 showed increase in cleaved caspase-3 expression. Neural progenitor cells from SVZ of miR-21 overexpressing mice cultured in vitro showed enhanced neurospheres proliferation and increased number of neurons following differentiation (Tuj1+ cells). Consistent with increased neurogenesis in the DG, miR-21 overexpressing mice exhibited enhanced learning in a Morris water maze task. Together, these results suggest that miR-21 plays a critical role in adult neurogenesis and hippocampus and has important effects on learning and memory. 

 

 

المؤتمر (2):

 

عنوان المؤتمر:

Neurogenesis during Development and in the Adult Brain 

تاريخ الإنعقاد:

  8-12 January 2017 

مكان الإنعقاد:

California, USA

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Investigating the role of the microRNA

miR-21 in Hippocampal Adult Neurogenesis 

ملخص المشاركة:

MicroRNAs (miRNAs) are a class of small non-coding RNAs that act as post-transcriptional regulators. Recent studies suggested that miRNAs play a role in neurodegenerative diseases and brain disorders (Fineberg, Kosik, & Davidson, 2009; Qureshi & Mehler, 2012). MiR-21, a miRNA that is dysregulated in cancers including glioblastomas, targets many cellular processes including cell proliferation and apoptosis cell proliferation (Si et al., 2007)and has been shown to be upregulated following post-traumatic brain injury and spinal cord injury to reduce lesion size, enhance cell survival and confer better neurological outcome after injury (Bhalala et al., 2012; Redell, Zhao, & Dash, 2011). Due to its effects on cell proliferation and survival, we speculate that miR-21 may play a role in adult neurogenesis in the mammalian brain. Here we investigate the effect of altering miR-21 levels on the cell fate of newborn neurons in the adult hippocampus. Using transgenic mice that globally either overexpress miR-21 or lack miR-21, we demonstrate that miR-21 overexpression led to increased neurogenesis in the dentate gyrus (DG), while its deletion showed a decreased neurogenesis in the same area. We show that miR-21 has a significant role in regulating hippocampal adult neurogenesis through altering cell survival. The decreased adult neurogenesis in the hippocampus led to impairment in learning and memory in the Morris water maze task. These results suggest that miR-21 plays an important role in hippocampal adult neurogenesis and therefore impacts on hippocampal-based learning and memory acquisition behaviours. 

 

 

 

المرفقالحجم
conference attendence letter 2_0.pdf‏124.74 ك.بايت
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