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الرئيسية / بندر بن عبدالمحسن العصيمي

بندر بن عبدالمحسن العصيمي

الأثنين _3 _مارس _2014

بندر بن عبدالمحسن العصيمي
دكتوراه
الطب والخدمات الصحية
University of Manchester

مجال التميز:

تميز دراسي وبحثي (ماجستير ودكتوراه)

البحوث المنشورة:

 

البحث (1):

 

عنوان البحث:

Increased Prevalence of JC Polyomavirus in Cervical Carcinomas from Women Infected with HIV

رابط البحث:

Click here

تاريخ النشر:

30/12/2013

موجز عن البحث:

Although subclinical persistent infections with the human polyomaviruses BKV and JCV are ubiquitous worldwide, these are known to vary in relation to diseases present and geographical location. DNAs from 220 cervical smears and 109 invasive cervical carcinomas obtained from HIV positive and HIV negative Kenyan women of known HPV status were analyzed by nested endpoint PCR for BKV and JCV. BKV–JCV DNA was detected in 5/105 (4.7%) of cervical smears and in 6/37 (16%) of cervical carcinomas from women infected with HIV whereas 9/115 (7.8%) of the cervical smears and 4/72 (5.5%) of the carcinomas were positive in HIV negative women. Nested PCR showed that all 24 samples were positive for JCV and not BKV. JCV was not more prevalent in either HPV positive (P¼0.438) or HPV negative women (P¼0.392). However, 37% of carcinomas and smears which were positive for JCV were also positive for a “high-risk” oncogenic HPV. Comparison of the incidence of JCV in cervical smears and cervical carcinomas showed a _3-fold increase in samples from HIV positive women with cervical carcinoma (P¼0.025) whereas no significant difference was found between cervical smears and cervical carcinomas from HIV negative women (P¼0.553). These results suggest that JCV may combine with high-risk HPV infection in women infected with HIV to influence the rate of progression to invasive cervical carcinoma.

المؤتمرات العلمية:

 

المؤتمر (1):

 

عنوان المؤتمر:

The 7th Saudi International Student Conference

تاريخ الإنعقاد:

1-2/02/2014

بلد ومكان الإنعقاد:

Edinburgh, UK

طبيعة المشاركة:

Paper presentation

ملخص المشاركة:

Background: The human polyomaviruses, BKV and JCV are ubiquitous in human populations worldwide. They sub-clinically establish persistent, latent infections and are capable of reactivating in a setting of immune suppression or immunodeficiency, leading to disease progression. We investigated the frequency of BKV and JCV existence in HIV-infected patients in comparison with HIV-negative patients.

Patients and Methods: A sample bank of 220 cervical smears from Kenyan women, in addition to 109 tissue biopsies from women with positive cervical carcinoma were analysed by a nested PCR for the large Tag gene of the sequences both BKV and JCV genomes.

Results: The overall prevalence of BKV-JCV DNA in cervical specimens was 24/329 (7.3%). BKV-JCV DNA was detected in 5/105 (4.7%) of cervical smears and in 6/37 (16%) of cervical carcinomas from women infected with HIV whereas 9/115 (7.8%) of the cervical smears and 4/72 (5.5%) of the carcinomas were positive in HIV negative women. Nested PCR showed that all 24 samples were positive for JCV and not BKV.

Conclusion: A correlation between the presence of JCV and immune status was found statistically significant (p= .025). Prevalence of JCV sequences was found 4 times higher with cervical tissue carcinomas in comparison to precancerous cervical smears in HIV-infected patients. JCV reactivation in a setting of immunodeficiency may associate with HPV infection to establish an etiological role in the progression to cervical cancer.

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