مجال التميز:

تميز
دراسي وبحثي (ماجستير ودكتوراه)

البحوث
المنشورة:

البحث
(1):

عنوان
البحث:

Increased
Prevalence of JC Polyomavirus in Cervical Carcinomas from Women Infected with
HIV

رابط
البحث:

Click
here

تاريخ
النشر:

30/12/2013

موجز
عن البحث:

Although
subclinical persistent infections with the human polyomaviruses BKV and JCV
are ubiquitous worldwide, these are known to vary in relation to diseases
present and geographical location. DNAs from 220 cervical smears and 109
invasive cervical carcinomas obtained from HIV positive and HIV negative
Kenyan women of known HPV status were analyzed by nested endpoint PCR for BKV
and JCV. BKV–JCV DNA was detected in 5/105 (4.7%) of cervical smears and in
6/37 (16%) of cervical carcinomas from women infected with HIV whereas 9/115
(7.8%) of the cervical smears and 4/72 (5.5%) of the carcinomas were positive
in HIV negative women. Nested PCR showed that all 24 samples were positive
for JCV and not BKV. JCV was not more prevalent in either HPV positive
(P¼0.438) or HPV negative women (P¼0.392). However, 37% of carcinomas and
smears which were positive for JCV were also positive for a “high-risk”
oncogenic HPV. Comparison of the incidence of JCV in cervical smears and
cervical carcinomas showed a _3-fold increase in samples from HIV positive
women with cervical carcinoma (P¼0.025) whereas no significant difference was
found between cervical smears and cervical carcinomas from HIV negative women
(P¼0.553). These results suggest that JCV may combine with high-risk HPV
infection in women infected with HIV to influence the rate of progression to
invasive cervical carcinoma.

المؤتمرات
العلمية:

المؤتمر
(1):

عنوان
المؤتمر:

The 7^th Saudi International Student Conference

تاريخ
الإنعقاد:

1-2/02/2014

بلد
ومكان الإنعقاد:

Edinburgh, UK

طبيعة
المشاركة:

Paper presentation

ملخص
المشاركة:

Background: The
human polyomaviruses, BKV and JCV are ubiquitous in human populations
worldwide. They sub-clinically establish persistent, latent infections and
are capable of reactivating in a setting of immune suppression or
immunodeficiency, leading to disease progression. We investigated the
frequency of BKV and JCV existence in HIV-infected patients in comparison
with HIV-negative patients.

Patients and
Methods: A sample
bank of 220 cervical smears from Kenyan women, in addition to 109 tissue
biopsies from women with positive cervical carcinoma were analysed by a
nested PCR for the large Tag gene of the sequences both BKV and JCV genomes.

Results: The overall prevalence of BKV-JCV DNA in
cervical specimens was 24/329 (7.3%). BKV-JCV DNA was detected in 5/105
(4.7%) of cervical smears and in 6/37 (16%) of cervical carcinomas from women
infected with HIV whereas 9/115 (7.8%) of the cervical smears and 4/72 (5.5%)
of the carcinomas were positive in HIV negative women. Nested PCR showed that
all 24 samples were positive for JCV and not BKV.

Conclusion: A correlation between the presence of JCV
and immune status was found statistically significant (p= .025). Prevalence
of JCV sequences was found 4 times higher with cervical tissue carcinomas in
comparison to precancerous cervical smears in HIV-infected patients. JCV
reactivation in a setting of immunodeficiency may associate with HPV
infection to establish an etiological role in the progression to cervical
cancer.