|
مجال التميز |
تميز دراسي و بحثي |
|
|
|
|
البحوث المنشورة |
|
|
البحث (1): |
|
|
عنوان البحث: |
Production, optimization, characterization and antifungal activity of chitinase produced by Aspergillus terrus |
|
رابط إلى البحث: |
|
|
تاريخ النشر: |
08/05/2014 |
|
موجز عن البحث: |
Chitin is one of the most abundant biopolymers widely distributed in the marine and terrestrial environments. Chitinase enzyme has received increased attention due to its wide range of biotechnological applications. Aspergillus terrus was found to be a good chitinase producer among the five fungi isolated from different soil samples from Al-Jouf city, Saudi Arabia. Maximum production of chitinase was obtained when using 2% of Shrimp-shell powder as a sole carbon source in the fermentation medium. The high level of chitinase production was observed in the culture medium with pH 5 at 30°C for five days at shaking conditions. Some properties of the crude chitinase were studied. In the present study, the antifungal activity of crude A. terrus chitinase was investigated against Apergillus niger, Aspergillus oryzae, Pencillum oxysporium, Rhizoctonia solani, Fusarium oxysporium, Rhizopus sp. and Mucor sp. and also estimated. The chitinase was found to inhibit the growth of some phytopathogenic fungi tested. The present work provides a suitable medium composition for enhancement of chitinase production by A. terrus and some properties of crude enzyme. Moreover, the study reflects the potential of A. terrus chitinase for biotechnological application. |
|
|
|
|
المؤتمرات العلمية: |
|
|
المؤتمر (1): |
|
|
عنوان المؤتمر: |
SEB(Society for Experimental Biology) Annual Meeting 2014 |
|
تاريخ الإنعقاد: |
01/07/2014 |
|
بلد ومكان الإنعقاد: |
Manchester. UK |
|
طبيعة المشاركة: |
Poster |
|
عنوان المشاركة: |
Roots, Soil and Phytoremediation. A fine-scale investigation of the potential of cereal roots in the phytoremediation of industrial phenolics. |
|
موجز عن المشاركة: |
The dynamic fine-scale heterogeneity of the root-soil interface (rhizosphere) provides many challenges for the experimentalist. An example of this is the investigation into the mechanisms that may provide valuable routes to the use of plants to clean up polluted environments (phytoremediation). Root function can vary at the sub-mm level as cells differentiate and mature along the axis. Simultaneously polluted soil can be heterogeneous and exhibit gradients at similar resolution. To describe and interpret this situation, we have applied techniques previously developed to study variation between individual plant cells (SiCSA; Single Cell Sampling and Analysis). Glass microcapillaries mounted on a micromanipulator are used to sample the moisture of soil microcosms in which plants are growing. Volumes of 2-3 nanolitres, occupying a soil volume of sub-mm dimensions, are isolated and analysed by capillary electrophoresis. Previous work has demonstrated the dynamic localised changes of organic acids and heavy metal in the rhizosphere. Organic pollutants of lower polarity, such as phenol, chlorophenol and nitrophenol offer different technical challenges. This poster presents the results of work in progress in this matter. (elpec9@bangor.ac.uk) |
المرفقات
- https://uksacb.org/wp-content/uploads/2014/07/certificate-of-Manchester-workshop2014_2.pdf
- https://uksacb.org/wp-content/uploads/2014/07/Farag-and-Al-Nusarie_2.pdf
- https://uksacb.org/wp-content/uploads/2014/07/certificate-of-Manchester-workshop2014_2.pdf
- https://uksacb.org/wp-content/uploads/2014/07/Farag-and-Al-Nusarie_2.pdf
