07/06/2023

حسين حسن خميس الدوسري

حسين حسن خميس الدوسري
دكتوراه
العلوم والتقنية
The University of East Anglia

مجال
التميز

تميز دراسي و بحثي

 

 

البحوث المنشورة

 

البحث (1):

 

عنوان البحث:

Proficiency of biosynthesized silver nanoparticles as a fungicide
against selected damping off causing fungi

رابط إلى البحث:

Click
Here

تاريخ النشر:

  01/07/2015

موجز عن البحث:

The present study aimed at
studying the fungicidal properties of nano-sized silver particles (AgNPs),
biosynthesized from Nocardiopsis dassonvillei at 10, 20, 40, 80 and 160 μg
ml-1 against selected damping off causing fungi. Silver nanoparticles (AgNPs)
were characterized by energy dispersive spectroscopy (EDS) and transmission
electron microscopy (TEM). TEM micrographs revealed extracellular formation
of spherical nanoparticles with an average size of 25.8 nm. All the tested
fungi were highly inhibited by the biosynthesized AgNPs. Its effective
concentrations inhibiting 50% of the fungal growth (EC50) values on F.
Solani, A. alternate, F. oxysporum and R. solani were 1.25, 3.23, 4.3 and 9.2
μg ml-1, respectively. The present study proved that biosynthesized AgNPs as
a promising fungicide against the treated fungi.

 

 

المؤتمرات العلمية:

 

المؤتمر (1):

 

عنوان المؤتمر:

The
7th Congress of European Microbiologists                  

تاريخ الإنعقاد:

  09/07/2017

مكان
الإنعقاد:

Valencia, Spain

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Responses of Soil Bacteria Communities to
Various Hydrocarbon Contaminants

ملخص المشاركة:

Hydrocarbons
contamination of soil is a widespread and urgent environmental problem. Many
studies have examined impacts of a single crude or fuel oil on microbial
community composition. Other studies have examined effects of one or more
polycyclic aromatic hydrocarbons (PAHs), but rarely at the same time as crude
or fuel oil. In consequence we do not know which components of oil have the
biggest effects on microbial communities in the soil. We compare responses of
bacteria communities to a linear alkane (hexadecane), a PAH (fluoranthene)
and diesel oil in different soil types, using Illumina sequencing of PCR
amplified bacterial 16S sequences supplemented with metagenomics data. There
were significant impacts of all hydrocarbons on the communities in both soil
types, with fluoranthene having the greatest effect.  The extent to which the
responses of communities in the two soils are similar depends upon the
taxonomic level at which the comparison is made, helping to explain reports
in the literature that different soils show idiosyncratic responses to
hydrocarbon contamination. 

 

 

 

المؤتمر (2):

 

عنوان المؤتمر:

Joint Annual Meeting: Ecology Across
Borders      

تاريخ الإنعقاد:

11/12/2017

مكان
الإنعقاد:

Ghent, Belgium

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Effects of Diesel contamination
on bacteria communities in various soil types

ملخص المشاركة:

Diesel contamination of soil is a
significant global problem. Microbial community composition changes in
response to hydrocarbons, but different soils often show contrasting
responses, and it is not known whether these differences are due to the
physical and chemical properties of the soil itself, or to initial taxonomic
composition. We investigate responses to diesel oil in three different soil
types and compare this with responses of the same communities inoculated into
(sterile) compost, using Illumina sequencing of PCR amplified bacterial 16S
sequences supplemented with metagenomic data. When data are analysed as
anonymous OTUs, corresponding approximately to “species”, all three soils are
different from each other. All show a response to diesel contamination, but
it is difficult to see any overarching pattern.  When phylogenetic relationships between
OTUs are taken into account using Unifrac distances, two soils show every
similar community composition and similar effects of diesel can be seen on
all three. Contamination must act on the taxa that are initially present, and
a substantial component of the idiosyncratic responses of different soils to
diesel oil contamination is because at a species level everything isn’t
everywhere.  The similarity of response
that emerges when phylogenetic relationships are accounted for will be
entirely invisible in older literature using fingerprinting techniques like
ARISA.

  

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