01/10/2023
  • السفارة

  • وزارة التعليم

  • سفير 2

  • الرئيسية

  • عن الملحقية

    • الملحق الثقافي

    • الملحقية

  • الأدلة والإجراءات

    • الدليل الشامل للمبتعث

    • دليل الطالب المالي

    • الدليل الطبي

    • اجراءات القنصلية

    • الدليل القانوني

      • التأشيرات

      • العقار – إنجلترا وويلز

  • الأخبار

    • أخبار

    • تعاميم

    • إعلانات

  • خدمات

    • سفير 2

    • العلاج

    • الأندية الطلابية

    • تصديق الشهادات والقبولات

    • تحميل الاستمارات

    • المكتبة الرقمية

  • إتصل بنا

  • الرئيسية

  • عن الملحقية

    • الملحق الثقافي

    • الملحقية

  • الأدلة والإجراءات

    • الدليل الشامل للمبتعث

    • دليل الطالب المالي

    • الدليل الطبي

    • اجراءات القنصلية

    • الدليل القانوني

      • التأشيرات

      • العقار – إنجلترا وويلز

  • الأخبار

    • أخبار

    • تعاميم

    • إعلانات

  • خدمات

    • سفير 2

    • العلاج

    • الأندية الطلابية

    • تصديق الشهادات والقبولات

    • تحميل الاستمارات

    • المكتبة الرقمية

  • إتصل بنا

نوره سلمان آل سماعيل

LnRiLWNvbnRhaW5lcltkYXRhLXRvb2xzZXQtYmxvY2tzLWNvbnRhaW5lcj0iNzA1MTliNzAwOTM0OThmOWM1OTFiNGUxNWIwMTUwNDgiXSB7IHBhZGRpbmc6IDI1cHg7Ym94LXNoYWRvdzogNXB4IDVweCAxMHB4IDAgcmdiYSggMCwgMCwgMCwgMC41ICk7Ym9yZGVyOiAwcHggc29saWQgcmdiYSggMCwgMCwgMCwgMSApOyB9IC50Yi1maWVsZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWZpZWxkPSIzYTA0MGYyNmJhY2NhMWYzM2E4Yzg4MjEzMTFkMjU5NiJdIGEgeyB0ZXh0LWRlY29yYXRpb246IG5vbmU7IH0gIC50Yi1ncmlkW2RhdGEtdG9vbHNldC1ibG9ja3MtZ3JpZD0iNTg1Y2MxZmUwMzA2MjM0YjI3MWJiZjlkZTA3YmNkNzEiXSB7IGdyaWQtdGVtcGxhdGUtY29sdW1uczogbWlubWF4KDAsIDAuMjk1ZnIpIG1pbm1heCgwLCAwLjcwNWZyKTtncmlkLWNvbHVtbi1nYXA6IDBweDtncmlkLXJvdy1nYXA6IDBweDtncmlkLWF1dG8tZmxvdzogcm93IH0gLnRiLWdyaWRbZGF0YS10b29sc2V0LWJsb2Nrcy1ncmlkPSI1ODVjYzFmZTAzMDYyMzRiMjcxYmJmOWRlMDdiY2Q3MSJdID4gLnRiLWdyaWQtY29sdW1uOm50aC1vZi10eXBlKDJuICsgMSkgeyBncmlkLWNvbHVtbjogMSB9IC50Yi1ncmlkW2RhdGEtdG9vbHNldC1ibG9ja3MtZ3JpZD0iNTg1Y2MxZmUwMzA2MjM0YjI3MWJiZjlkZTA3YmNkNzEiXSA+IC50Yi1ncmlkLWNvbHVtbjpudGgtb2YtdHlwZSgybiArIDIpIHsgZ3JpZC1jb2x1bW46IDIgfSAudGItZ3JpZC1jb2x1bW5bZGF0YS10b29sc2V0LWJsb2Nrcy1ncmlkLWNvbHVtbj0iMzA5MDQ3NzVkOTA5N2Q5NzQ0OTFkNDE3M2Q2YTcyM2IiXSB7IGRpc3BsYXk6IGZsZXg7IH0gLnRiLWdyaWQtY29sdW1uW2RhdGEtdG9vbHNldC1ibG9ja3MtZ3JpZC1jb2x1bW49IjMwOTA0Nzc1ZDkwOTdkOTc0NDkxZDQxNzNkNmE3MjNiIl0gcCB7IGZvbnQtc2l6ZTogMzZweDtsaW5lLWhlaWdodDogMzFweDt0ZXh0LXRyYW5zZm9ybTogdXBwZXJjYXNlOyB9IC50Yi1maWVsZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWZpZWxkPSIwNTkwMTY0NDc2OTlmMmY0MjZkNDhiNjFhODVhMDYyMiJdIHsgZm9udC1zaXplOiAyNnB4O2xpbmUtaGVpZ2h0OiA0MnB4O3RleHQtYWxpZ246IHJpZ2h0O3BhZGRpbmctbGVmdDogMTVweDsgfSAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iMDU5MDE2NDQ3Njk5ZjJmNDI2ZDQ4YjYxYTg1YTA2MjIiXSBhIHsgdGV4dC1kZWNvcmF0aW9uOiBub25lOyB9ICAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iYjQ4ZTZiMzgzZGNmY2RkNzVhOTQ1MzMxOTk5ZmI2ZDYiXSB7IGZvbnQtc2l6ZTogMjhweDtsaW5lLWhlaWdodDogMjhweDt0ZXh0LWFsaWduOiByaWdodDtwYWRkaW5nLWxlZnQ6IDE1cHg7IH0gLnRiLWZpZWxkW2RhdGEtdG9vbHNldC1ibG9ja3MtZmllbGQ9ImI0OGU2YjM4M2RjZmNkZDc1YTk0NTMzMTk5OWZiNmQ2Il0gYSB7IHRleHQtZGVjb3JhdGlvbjogbm9uZTsgfSAgLnRiLWZpZWxkW2RhdGEtdG9vbHNldC1ibG9ja3MtZmllbGQ9ImNjOGIwNzI3MzFkYWI0YmU5ZDNkNTBlZjVlZDdjYzA2Il0geyBmb250LXNpemU6IDI4cHg7bGluZS1oZWlnaHQ6IDI4cHg7dGV4dC1hbGlnbjogcmlnaHQ7cGFkZGluZy1sZWZ0OiAxNXB4OyB9IC50Yi1maWVsZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWZpZWxkPSJjYzhiMDcyNzMxZGFiNGJlOWQzZDUwZWY1ZWQ3Y2MwNiJdIGEgeyB0ZXh0LWRlY29yYXRpb246IG5vbmU7IH0gIC50Yi1ncmlkLWNvbHVtbltkYXRhLXRvb2xzZXQtYmxvY2tzLWdyaWQtY29sdW1uPSJhOTQ3N2IzYjJhY2JhYjVlODE3YWUyMWNjNmQwOTczZSJdIHsgZGlzcGxheTogZmxleDsgfSBbZGF0YS10b29sc2V0LWJsb2Nrcy1pbWFnZT0iZWYzOGFiN2M0MzY3OWVlZDkwODdlZTQzYjU4MjkxMDUiXSB7IG1heC13aWR0aDogMTAwJTsgfSBbZGF0YS10b29sc2V0LWJsb2Nrcy1pbWFnZT0iZWYzOGFiN2M0MzY3OWVlZDkwODdlZTQzYjU4MjkxMDUiXSAudGItaW1hZ2Utc2hhZG93LTEtaW5uZXIgeyBib3JkZXI6IDBweCBzb2xpZCByZ2JhKCAwLCAwLCAwLCAwLjI1ICk7Ym9yZGVyLXJhZGl1czogMDsgfSBAbWVkaWEgb25seSBzY3JlZW4gYW5kIChtYXgtd2lkdGg6IDc4MXB4KSB7IC50Yi1maWVsZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWZpZWxkPSIzYTA0MGYyNmJhY2NhMWYzM2E4Yzg4MjEzMTFkMjU5NiJdIGEgeyB0ZXh0LWRlY29yYXRpb246IG5vbmU7IH0gIC50Yi1ncmlkW2RhdGEtdG9vbHNldC1ibG9ja3MtZ3JpZD0iNTg1Y2MxZmUwMzA2MjM0YjI3MWJiZjlkZTA3YmNkNzEiXSB7IGdyaWQtdGVtcGxhdGUtY29sdW1uczogbWlubWF4KDAsIDAuNWZyKSBtaW5tYXgoMCwgMC41ZnIpO2dyaWQtYXV0by1mbG93OiByb3cgfSAudGItZ3JpZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWdyaWQ9IjU4NWNjMWZlMDMwNjIzNGIyNzFiYmY5ZGUwN2JjZDcxIl0gPiAudGItZ3JpZC1jb2x1bW46bnRoLW9mLXR5cGUoMm4gKyAxKSB7IGdyaWQtY29sdW1uOiAxIH0gLnRiLWdyaWRbZGF0YS10b29sc2V0LWJsb2Nrcy1ncmlkPSI1ODVjYzFmZTAzMDYyMzRiMjcxYmJmOWRlMDdiY2Q3MSJdID4gLnRiLWdyaWQtY29sdW1uOm50aC1vZi10eXBlKDJuICsgMikgeyBncmlkLWNvbHVtbjogMiB9IC50Yi1ncmlkLWNvbHVtbltkYXRhLXRvb2xzZXQtYmxvY2tzLWdyaWQtY29sdW1uPSIzMDkwNDc3NWQ5MDk3ZDk3NDQ5MWQ0MTczZDZhNzIzYiJdIHsgZGlzcGxheTogZmxleDsgfSAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iMDU5MDE2NDQ3Njk5ZjJmNDI2ZDQ4YjYxYTg1YTA2MjIiXSBhIHsgdGV4dC1kZWNvcmF0aW9uOiBub25lOyB9ICAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iYjQ4ZTZiMzgzZGNmY2RkNzVhOTQ1MzMxOTk5ZmI2ZDYiXSBhIHsgdGV4dC1kZWNvcmF0aW9uOiBub25lOyB9ICAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iY2M4YjA3MjczMWRhYjRiZTlkM2Q1MGVmNWVkN2NjMDYiXSBhIHsgdGV4dC1kZWNvcmF0aW9uOiBub25lOyB9ICAudGItZ3JpZC1jb2x1bW5bZGF0YS10b29sc2V0LWJsb2Nrcy1ncmlkLWNvbHVtbj0iYTk0NzdiM2IyYWNiYWI1ZTgxN2FlMjFjYzZkMDk3M2UiXSB7IGRpc3BsYXk6IGZsZXg7IH0gIH0gQG1lZGlhIG9ubHkgc2NyZWVuIGFuZCAobWF4LXdpZHRoOiA1OTlweCkgeyAudGItZmllbGRbZGF0YS10b29sc2V0LWJsb2Nrcy1maWVsZD0iM2EwNDBmMjZiYWNjYTFmMzNhOGM4ODIxMzExZDI1OTYiXSBhIHsgdGV4dC1kZWNvcmF0aW9uOiBub25lOyB9ICAudGItZ3JpZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWdyaWQ9IjU4NWNjMWZlMDMwNjIzNGIyNzFiYmY5ZGUwN2JjZDcxIl0geyBncmlkLXRlbXBsYXRlLWNvbHVtbnM6IG1pbm1heCgwLCAxZnIpO2dyaWQtYXV0by1mbG93OiByb3cgfSAudGItZ3JpZFtkYXRhLXRvb2xzZXQtYmxvY2tzLWdyaWQ9IjU4NWNjMWZlMDMwNjIzNGIyNzFiYmY5ZGUwN2JjZDcxIl0gID4gLnRiLWdyaWQtY29sdW1uOm50aC1vZi10eXBlKDFuKzEpIHsgZ3JpZC1jb2x1bW46IDEgfSAudGItZ3JpZC1jb2x1bW5bZGF0YS10b29sc2V0LWJsb2Nrcy1ncmlkLWNvbHVtbj0iMzA5MDQ3NzVkOTA5N2Q5NzQ0OTFkNDE3M2Q2YTcyM2IiXSB7IGRpc3BsYXk6IGZsZXg7IH0gLnRiLWZpZWxkW2RhdGEtdG9vbHNldC1ibG9ja3MtZmllbGQ9IjA1OTAxNjQ0NzY5OWYyZjQyNmQ0OGI2MWE4NWEwNjIyIl0gYSB7IHRleHQtZGVjb3JhdGlvbjogbm9uZTsgfSAgLnRiLWZpZWxkW2RhdGEtdG9vbHNldC1ibG9ja3MtZmllbGQ9ImI0OGU2YjM4M2RjZmNkZDc1YTk0NTMzMTk5OWZiNmQ2Il0gYSB7IHRleHQtZGVjb3JhdGlvbjogbm9uZTsgfSAgLnRiLWZpZWxkW2RhdGEtdG9vbHNldC1ibG9ja3MtZmllbGQ9ImNjOGIwNzI3MzFkYWI0YmU5ZDNkNTBlZjVlZDdjYzA2Il0gYSB7IHRleHQtZGVjb3JhdGlvbjogbm9uZTsgfSAgLnRiLWdyaWQtY29sdW1uW2RhdGEtdG9vbHNldC1ibG9ja3MtZ3JpZC1jb2x1bW49ImE5NDc3YjNiMmFjYmFiNWU4MTdhZTIxY2M2ZDA5NzNlIl0geyBkaXNwbGF5OiBmbGV4OyB9ICB9IA==
نوره سلمان آل سماعيل
دكتوراه
الطب والخدمات الصحية
University of Surrey

مجال التميز

تميز دراسي وبحثي 

البحوث المنشورة

 

البحث (1):

 

عنوان البحث:

Activation of the Farnesoid X-receptor in breast cancer cell lines results in cytotoxicity but not increased migration potential

رابط إلى البحث:

Click here

تاريخ النشر:

03/11/2015

موجز عن البحث:

Breast cancer is the commonest form of cancer in women, but successful treatment is confounded by the heterogeneous nature of breast tumours: Effective treatments exist for hormone-sensitive tumours, but triple-negative breast cancer results in poor survival. An area of increasing interest is metabolic reprogramming, whereby drug-induced alterations in the metabolic landscape of a tumour slow tumour
growth and/or increase sensitivity to existing therapeutics. Nuclear receptors are transcription factors central to the expression of metabolic and transport proteins, and thus represent potential targets for metabolic reprogramming. We show that activation of the nuclear receptor FXR, either by its endogenous
ligand CDCA or the synthetic GW4064, leads to cell
death in four breast cancer cell lines with distinct phenotypes: MCF-10A (normal), MCF-7 (receptor
positive), MDA-MB-231 and MDA-MB-468 (triple negative). Furthermore, we show that the mechanism of cell death is predominantly through the intrinsic
apoptotic pathway. Finally, we demonstrate that FXR agonists do not stimulate migration in breast cancer cell lines
, an important potential adverse effect.
Together, our data support the continued examination of FXR agonists as a novel class of therapeutics for the treatment of breast cancer.

المؤتمرات العلمية:

 

المؤتمر (1):

 

عنوان المؤتمر:

8th Saudi Students Conference

تاريخ الإنعقاد:

31/01-01/02/2015

مكان الإنعقاد:

London, UK

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Farnesoid X Receptor role on modulation of Matrix Metalloproteinases -2 and -9 in Metastatic Breast Cancer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ملخص المشاركة:

Advanced breast carcinoma is a main cause of mortality affecting women. It is associated with poor prognosis; therefore, understanding the molecular mechanisms of invasive malignancies is critical in order to discover new therapies. The aim of the current study was to investigate whether Farnesoid X receptor (FXR); a nuclear receptor which is highly expressed in Breast carcinoma is a novel regulator of matrix metalloprotease-2 and -9 (MMP-2 and-9) that play main role in breast cancer metastasis, and their endogenous inhibitors, Tissue inhibitors of metalloproteases (TIMPs). TIMP-2 and-1 inhibit MMP-2 and-9 respectively. Hence, FXR may represent a novel therapeutic target. Two FXR agonists were used to measure their effects on breast cancer cells MDA-MB-231, MDA-MB-468 (triple negative), MCF-7 (Estrogen receptor positive) and normal cells MCF10A: CDCA is an endogenous, while GW4064 is a synthetic ligand. Cell viability, protein and mRNA levels of matrix metalloprotease -2 and -9 and TIMP-2 and-1, and cell migration were assessed using both molecular and
cellular techniques. Both FXR agonists decreased breast cancer and normal breast cell viability, with the effects more significant in the triple negative cells, suggesting a potential targeting towards aggressive cancer.
However, the FXR ligands didn’t alter mRNA and protein levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 intracellularlly or extracellularlly, suggesting that this cytotoxic effect may not be via MMP. FXR ligands also had no effect on breast cancer migration, which is consistent with the suggestion that FXR activation has a general cytotoxic effect on tumour cells, but does not
directly impact on tumour migration. In conclusion, FXR activation doesn’t impact on markers of breast cancer metastasis, suggesting that its potential as a therapeutic target to prevent tumour progression may be limited.
However, the cytotoxic effect on cancer cells is promising, suggesting that these agonists may still possess some potential for breast cancer therapy.

المؤتمر (2):

 

عنوان المؤتمر:

7th Saudi Students Conference

تاريخ الإنعقاد:

1-2/02/2014

مكان الإنعقاد:

Edinburgh, UK 

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Role of Farnesoid X Receptor in Regulation of Metastatic Breast Cancer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ملخص المشاركة:

Breast cancer is a primary cause of mortality in women and it is correlated to poor prognosis. Therefore,
understanding the molecular mechanisms of invasive cancers is
fundamental for the discovery of new generation of treatments. Degradation of extracellualr matrix is required for the malignancies to invade and migrate to distant organs, and that occurs by specific endopeptidases which are called Matrix metalloproteinases (MMPs) with high expression of MMP-2 and -9 in metastatic breast cancer in particular. MMP activity is tightly regulated by the tissue inhibitors of metalloproteinases (TIMPs). Researchers have shown that the nuclear receptor Farnesoid X-receptor (FXR) is involved in regulation of MMP and TIMP activity in hepatic and vascular tissues. The rationale of the current study was to investigate whether FXR is a novel regulator of MMP-2 and -9 as it is highly expressed in metastatic breast cancer. Initially the viability of the breast cancer cell lines MCF7 (Estrogen receptor positive) and MDA-MB-468 (The triple negative) was measured after exposure to the FXR agonists chenodeoxycholic acid (CDCA) and 3-[2-[2-Chloro-4-[[3-(2, 6 dichlorophenyl)-5-(1-methylethyl)-4
isoxazolyl]methoxy]phenyl]ethenyl]benzoic acid (GW4064).
Our findings showed that FXR ligands caused cell death and the effects were more significant in the triple negative cells. Protein and mRNA levels of MMP-2 and -9 within both cell lines did not change after FXR activation, when measured by Western blotting and real time PCR. Furthermore, the migratory response and the effect on activity of MMP for FXR ligands are being investigated. Our finding suggests
that FXR activation is not pro-metastatic, but can kill tumour cells, so it might be a novel therapeutic target for metastatic breast cancer.

 

 

المؤتمر (3):

6th Saudi Students Conference

عنوان المؤتمر:

11-14 /10 /2012

تاريخ الإنعقاد:

London, UK

مكان الإنعقاد:

Poster presentation

طبيعة المشاركة:

FXR Regulates Matrix Metalloproteinases in Breast Cancer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

عنوان المشاركة:

Metastasis is associated with poor prognosis in breast cancer. Thus it is crucial to understand the molecular mechanisms of tumour cell invasion and metastasis in
order to identify therapeutic targets. Metastasis requires degradation of the extracellular matrix (ECM), permitting cancer cells to invade and spread to distant sites. Matrix metalloproteinases (MMPs) are essential for degrading the proteins of the ECM. In particular, MMP-2 and -9 expression correlates with poor breast cancer prognosis. MMP activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). TIMP-1 inhibits MMP-9 whereas TIMP-2 inhibits MMP-2. Studies have shown that the nuclear receptor Farnesoid X receptor (FXR) is involved in MMP and TIMP regulation in hepatic and vascular tissues and is highly expressed in breast cancer.

So the aim of this project was to investigate whether FXR is a novel regulator of MMP-2 and -9 in breast cancer cells.
Initially the
cell viability of breast cancer cell lines MCF7 (Estrogen receptor positive) and MDA-MB-468 (Estrogen receptor negative) after treatment with FXR agonists CDCA and GW4064 were measured. FXR ligands decreased the cell viability in both cell lines and the effects were more significant in MDA-MB-468. The mRNA and protein levels of MMP-2 and -9 within both cell lines did not change after FXR activation, when measured by real time PCR and Western blotting. Interestingly, when the activity of the MMP-2 and -9 enzymes secreted into the culture media was measured using a fluorescent substrate; GW4064 increased MMP activity in a concentration dependant manner, whereas CDCA had no effect. To determine whether this was due to FXR regulation of TIMPs, their mRNA expression was measured by real time PCR. There was no effect of FXR ligands on TIMP-1 and -2 mRNA levels.  

In conclusion, FXR activation by GW4064 increases secreted MMP-2 and -9 activity in breast cancer cells but this effect is not via transcriptional regulation of the MMPs or their inhibitory factors TIMP-1 or -2.

المؤتمر (4):

 

عنوان المؤتمر:

International Society for the Study of Xenobiotics (ISSX)

تاريخ الإنعقاد:

17/06/2012

مكان الإنعقاد:

Noordwijjk, Netherlands

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

Regulation Of Matrix Metalloproteinases By FXR In Metastatic Breast Cancer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ملخص المشاركة:

Metastasis is associated with poor prognosis in breast cancer. Thus it is crucial to understand the molecular mechanisms of tumour cell invasion and metastasis in order to identify therapeutic targets. Metastasis requires degradation of the extracellular matrix (ECM), permitting cancer cells to invade and spread to distant sites. Matrix metalloproteinases (MMPs) are essential for degrading the proteins of the ECM. In particular, MMP-2 and -9 expression correlate with poor breast cancer prognosis. MMP activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). TIMP-1
inhibits MMP-9 whereas TIMP-2 inhibits MMP-2. Studies have shown that the nuclear receptor Farnesoid X receptor (FXR) is involved in MMP and TIMP regulation in hepatic and vascular tissues and is highly expressed in breast cancer1-3. So the aim of this project was to investigate whether FXR is a novel regulator of MMP-2 and -9 in breast cancer cells. Initially the cell viability of breast cancer cell lines MCF7 (Estrogen receptor positive) and MDA-MB-468 (Estrogen receptor negative) after treatment with FXR agonists chenodeoxycholic acid (CDCA) and3-[2-[2-Chloro-4-[[3-(2,6-dichlorophenyl)-5-(1-methylethyl)-4
isoxazolyl]methoxy]phenyl]ethenyl]benzoic acid, known as GW4064 were measured. FXR ligands decreased the cell viability in both cell lines and the effects were more significant in MDA-MB-468. The mRNA and protein levels of MMP-2 and -9 within both cell lines did not change after FXR activation, when measured by real time PCR and Western blotting.  Interestingly, when the activity of the MMP-2  and -9 enzymes secreted into the culture media was measured using a fluorescent substrate; GW4064 increased MMP activity in a concentration dependant manner, whereas CDCA had no effect. To determine whether this was due to FXR regulation of TIMPs, their mRNA expression was measured by real time PCR. There was no effect of FXR ligands on TIMP-1 and -2 mRNA levels. In conclusion, FXR activation by GW4064 increases secreted MMP-2 and -9 activity in breast cancer cells but this effect is not via transcriptional regulation of the MMPs or their inhibitory factors TIMP-1 or -2.

المؤتمر (5):

 

عنوان المؤتمر:

5th Saudi Students Conference

تاريخ الإنعقاد:

23-26/06/2011

مكان الإنعقاد:

Coventry, UK

طبيعة المشاركة:

Poster presentation

عنوان المشاركة:

The Role of Farnesoid X Receptor in Matrix Metalloproteinase Regulation in Breast Cancer

 

Breast cancer is a leading cause of female death in the UK. Therefore, developing new drugs is crucial. The nuclear receptor FXR is a potential novel therapeutic target. FXR is activated by bile acids, is expressed and is pro-apoptotic in breast cancers. Matrix
metalloproteinases (MMP) 9 & 2 are enzymes involved in tissue remodelling, key to invasion and metastasis of breast tumours. Could FXR be a novel regulator of MMP-2 and -9? Initial experiments were performed to establish the effects of FXR ligands on the cell viability of breast cancer cell lines MCF7 (Estrogen receptor positive) and MDA-MB-468 (Estrogen receptor negative). The cells were 100% viable in the vehicle control.  For MDA-MB-468, CDCA decreased cell viability to 80 & 59% (10% serum conditions) and 62 & 33% (serum free conditions) for 24h and 48h exposures respectively. In contrast, GW4064 only
decreased MDA-MB-468 viability under serum free conditions, to 75% (24h) & 41% (48h).  Similarly, CDCA
decreased MCF7 cell viability to almost 86%, under both serum and serum free conditions at 24 and 48h time points. Whereas GW4064 only decreased MCF7
viability to 76% after 48h under serum-free conditions. These effects on cell viability need to be considered during investigations into MMP regulation.
MMP-2
&-9 activity were measured using a fluorescent substrate in conditioned media collected from the cell viability experiments.  GW4064 increased, whereas CDCA decreased MMP activity in a concentration dependant manner. We are now investigating these opposing results further. In addition we are measuring the protein and mRNA levels of MMP-2 and -9 by Western blot and real-time PCR.


المرفقات

  • https://uksacb.org/wp-content/uploads/2015/11/ISSX-conference-.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference-2012.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference-2014.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference2011.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/SIC-2015.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/ISSX-conference-.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference-2012.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference-2014.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/Saudi-conference2011.pdf
  • https://uksacb.org/wp-content/uploads/2015/11/SIC-2015.pdf
  • تويتر
  • فيسبوك
  • بنترست

آخر الأخبار

  • الملحقية الثقافية تعقد ورشة عمل توعوية قانونية للمبتعثين الجدد
    1 أكتوبر، 2023
  • الملحقية الثقافية تنظم حفل بمقرها في العاصمة البريطانية لندن بمناسبة اليوم الوطني السعودي٩٣
    28 سبتمبر، 2023
  • سمو سفير خادم الحرمين الشريفين لدى المملكة المتحدة يستقبل وزير التعليم
    20 سبتمبر، 2023

آخر التعاميم والإعلانات

  • دعوة لحضور حفل اليوم الوطني السعودي ٩٣ وزيارة معرض نحلم ونحقق٩٣
    20 سبتمبر، 2023
  • تعميم أجازة
    20 سبتمبر، 2023
  • اللقاء التوعوي القانوني للمبتعثين الجدد ٢٠٢٣
    19 سبتمبر، 2023

أوقات الدوام

  • الإثنين:    09 صباحاً – 4 مساء
  • الثلاثاء:    09 صباحاً – 4 مساءً
  • الأربعاء:   09 صباحاً – 4 مساءً
  • الخميس:  09 صباحاً – 4 مساءً
  • الجمعة:   09 صباحاً – 4 مساءً

الملحقية الثقافية السعودية

عناوين الملحقية


sacbuk@uk.moe.gov.sa


+44 20 3249 7000


630 Chiswick High Road London W4 5RY

  • دليل الدارس في المملكة المتحدة

  • تصديق الشهادات والقبولات

  • العلاج الطبي

  • إتصل بنا

© 2023 | بتقنية قالب مهارتي