مجال التميز |
تميز دراسي وبحثي |
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البحوث المنشورة |
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البحث (1): |
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عنوان البحث: |
Rise In Group W Meningococcal Carriage In University Students, United Kingdom |
رابط إلى البحث: |
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تاريخ النشر: |
19/05/2017 |
موجز عن البحث: |
MenACWY conjugate vaccination was recently introduced in the United Kingdom for adolescents and young adults to reduce disease from infection by Neisseria meningitidis group W. We conducted a cross-sectional meningococcal carriage study in first-year UK university students. Despite 71% MenACWY vaccine coverage, carriage of group W increased substantially. |
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المؤتمرات العلمية: |
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المؤتمر (1): |
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عنوان المؤتمر: |
Microbiology Society Annual Conference |
تاريخ الإنعقاد: |
3-6 April 2017 |
مكان الإنعقاد: |
Edinburgh, United Kingdom |
طبيعة المشاركة: |
Poster presentation |
عنوان المشاركة: |
Investigating The Mechanisms Of Secretion Of Moonlighting Proteins Enolase And FBA In Neisseria Meningitidis |
ملخص المشاركة: |
Neisseria meningitidis is an obligate commensal of humans that colonizes the nasopharynx. It is also an important cause of serious diseases such as meningitis and sepsis. Several secretion systems have been shown to operate in meningococci, which enable the bacteria to modulate their cell surface and evade the human immune system. There is an increasing awareness of the contribution of moonlighting proteins to bacterial pathogenesis; these are secreted via unknown secretion mechanisms. The moonlighting proteins FBA and enolase were previously shown to localize to the surface of N. meningitidis. The mechanisms of secretion of both proteins were investigated in this study since the pathway of their translocation into meningococcal cell surface remains unclear. Specifically, the lysine residue 337 of meningococcal enolase was mutated by site-directed mutagenesis to investigate its role in enolase secretion since in Escherichia coli mutation of the corresponding residue has been reported to decrease export efficiency. The production of N. meningitidis MC58 rpsLR strains containing either wild type or mutated ectopic versions of the enolase was successful. Unmarked mutants lacking the native enolase gene using the two complemented strains were also successfully generated. Finally, whole-cell ELISA was optimised to investigate the contribution of several secretion-related or pathogenesis-related proteins to FBA and enolase surface localization. This analysis showed that the mutation of pilQ, porA, skp, surA, hlyB, and cbbAEctK354A did not significantly affect the amount of FBA on the cell surface. |
بنان عدنان يحي عطوه
دكتوراه
الطب والخدمات الصحية
University of Nottingham