المؤتمرات العلمية:
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المؤتمر (1):
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عنوان المؤتمر:
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TERMIS World Congress 2012
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تاريخ الإنعقاد:
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September 2012
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بلد ومكان الإنعقاد:
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Vienna, Austria
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طبيعة المشاركة:
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عرض ورقة علمية
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عنوان المشاركة:
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Potential Role for Aligned Fibre Scaffolds in
Periodontal Tissue Engineering
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موجز عن المشاركة:
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Periodontal disease is a chronic inflammatory process characterized by
destruction and loss of connective and mineralised tissues. Current
therapeutic techniques may not predictably result in full regeneration of the
lost periodontal structures. A tissue engineering approach may have the
potential to fully regenerate the periodontal tissues. The aim of the study
was investigate the effect of fibre alignment of electrospun poly-L-lactic
acid (PLLA) mats on the biological response of periodontal ligament
fibroblasts (PDLFs). An 8% PLLA polymer solution was electrospun to fabricate
aligned scaffolds. Random PLLA fibre scaffolds were fabricated as a control.
PDLFs were seeded onto the PLLA scaffolds and cultured for varying periods of
time with and without mechanical loading. The resultant constructs were
analysed by scanning electron microscopy (SEM), confocal microscopy,
immunohistochemistry and q-PCR. The alignment of the electrospun PLLA fibres
was confirmed by SEM. Good attachment and proliferation of PDLFs on both
scaffold type was observed. On aligned scaffolds, PDLFs were elongated in
shape and oriented along the long-axis of the fibres and showed a higher
level of cellular activity. Immunohistochemical and gene expression analysis
indicated that fibre alignment can influence the phenotype of periodontal
ligament fibroblasts. In conclusion, aligned fibre matrices may be suitable
candidates for periodontal tissue engineering.
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المؤتمر (2):
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عنوان المؤتمر:
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TERMIS EU 2011 Annual Meeting
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تاريخ الإنعقاد:
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June 2011
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بلد ومكان الإنعقاد:
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Granada, Spain
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طبيعة المشاركة:
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عرض ورقة علمية
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عنوان المشاركة:
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Electrospun Poly-L-Lactic Acid Aligned Scaffolds for
Periodontal Ligament
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موجز عن المشاركة:
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Periodontal disease is a chronic disease involves
periodontal ligament and bone, which is characterized by gingival
inflammation and periodontal attachment loss, eventually leading to tooth
loss (Armitage, 2004).
Regeneration of the lost periodontal tissue is the
ultimate goal of periodontal treatment. A variety of procedures including
guided tissue regeneration and root surface application of various growth
factors as well as bone and enamel matrix proteins has been used to treat
periodontal defects. However, the amount of periodontal tissue regenerated
utilizing these treatment approaches is not predictable and site specific.
Periodontal tissue engineering is a relatively new
field with enormous potential to fully regenerate the lost periodontal
tissues and overcome some of the limitation in the existing periodontal
therapies. The aim of periodontal tissue engineering is to develop procedures
and biomaterials to create a periodontal tissue graft ex vivo and implant it
in vivo for full periodontal regeneration. It involves three basic components
namely: responsive progenitor cells, signalling molecules, and a suitable
scaffold support for tissue growth.
In this study we investigate different periodontal
cell sources and different scaffolds for periodontal tissue engineering.
Recently, much interest has been directed towards the construction of aligned
biomaterial scaffolds. Aligned fibre scaffolds reflect the general
topographic arrangement of collagen fibres of the native extracellular matrix
of periodontal ligament which may influence cell behaviour
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المؤتمر (3):
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عنوان المؤتمر:
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British Society of Periodontology
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تاريخ الإنعقاد:
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May 2011
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بلد ومكان الإنعقاد:
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Belfast, UK
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طبيعة المشاركة:
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عرض ملصق
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عنوان المشاركة
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Natural and synthetic aligned fibre matrices as
potential scaffolds for periodontal tissue engineering
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موجز عن المشاركة
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Aim:
The aims of the study
were
1.to fabricate aligned
electrospun poly-L-lactic acid (PLLA) scaffolds
2.to decellularised
bovine ligament as naturally aligned collagen matrices
3.to investigate the
response of periodontal ligament fibroblasts to these biomaterials (PDLF).
Method:
PLLA solution was
prepared and electrospun to produce both aligned and random scaffolds.
Specimens of bovine ligaments were decellularised and assessed using DNA
quantification assay and nuclear staining. PDLFs were seeded onto the
scaffolds and cultured for different periods. The resultant constructs were
analysed by scanning electron microscopy (SEM), confocal microscopy (CFM) and
immunohistochemistry (IHC).
Result:
SEM confirmed alignment of PLLA fibres and PDLFs were
observed to attach and proliferate. Actin filament staining showed that the
cells were oriented and elongated along the long axis of the fibres. IHC
showed greater staining density for collagen I and periostin in the aligned
fibre constructs. This indicates that fibre alignment may exert an effect on
cell phenotype of PDLF. SEM micrographs of decellularised ligament showed
that morphology was maintained throughout the decellularisation process and
PDLF were oriented along the long axis of the collagen fibres.
Conclusion:
Aligned fibre matrices
such as electrospun PLLA aligned scaffolds and decellularised natural
ligaments may be suitable candidates for periodontal tissue engineering.
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المؤتمر (4):
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عنوان المؤتمر:
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The Fifth Saudi International
Conference (SIC2011)
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تاريخ الإنعقاد:
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June 2011
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بلد ومكان الإنعقاد:
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The University of
Warwick, UK
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طبيعة المشاركة:
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تكريم
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المؤتمر (5):
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عنوان المؤتمر:
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UK Society for Biomaterial 2010
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تاريخ الإنعقاد:
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July 2010
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بلد ومكان الإنعقاد:
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Glasgow, UK
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طبيعة المشاركة:
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عرض ملصق
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عنوان المشاركة
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Electrospun poly-L-lactic acid aligned scaffolds
for periodontal ligament regeneration, mimicking native tissue
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موجز عن المشاركة
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Periodontal disease is a chronic
inflammatory process characterized by destruction and loss of connective and
mineralised tissues. The therapeutic goals are to eliminate inflammation,
restore ligament attachment, and increase bone volume. Tissue-engineering may
overcome the limitations associated with current treatments. Synthetic
fibrous scaffolds for tissue engineering may be produced by
electrospinning. Much interest has
been directed towards the construction of aligned scaffolds, as this feature
may influence cell behaviour. Aligned fibre scaffolds should be particularly
suitable for regeneration of the periodontal ligament as they more closely
resemble this property in healthy native extracellular matrix.
The aim of this study was to
fabricate aligned scaffolds and investigate the response of periodontal
ligament fibroblasts (PDLFs) to these biomaterials
To prepare aligned scaffolds, an
8% (w/v) poly-L-lactic acid (PLLA) solution was made by dissolving PLLA
granules in dichloromethane (DCM). Before electrospinning, the PLLA solution
was fed into a plastic syringe, which was controlled by a syringe pump at a
feeding rate of 2 ml/h. A rotating collector (a motorized take-up mandrel)
was used to obtain the aligned fibre structures. During electrospinning, a
voltage of 18 kV was applied on the nozzle to generate a stable polymer jet.
Random PLLA fibre scaffolds were used as a control.
The scaffold, produced by
electrospinning, was a nonwoven, three-dimensional, and highly-aligned
fiber-based matrix. The metabolic activity of the PDLFs on all scaffolds
increased over time. The metabolic activity of the cells on aligned fibres
was significantly higher at 4 hours incubation, compared to the random
scaffolds (Figure-4). Scanning electron images showed PDLFs on the aligned
electrospun fibres were elongated along the fibre axis in a spindle shape.
Conversely, cells on random scaffolds grew in a polygonal shape with no
orientation (Figure-5).
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جوائز التكريم
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الجائزة (1):
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مسمى الجائزة
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2nd Best Paper Award in
Medical Science
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الجهة المانحة:
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The Fifth Saudi International Conference (SIC2011)
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تاريخ منح الجائزة:
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June 2011
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مجال التكريم:
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تميز بحثي
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