مجال التميز | بحثي دراسي |
البحوث المنشورة |
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البحث (1): | |
عنوان البحث: | Inflammation associated ethanolamine facilitates infection by Crohn’s disease-linked adherent-invasive Escherichia coli |
رابط إلى البحث: | https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(19)30216-6/fulltext |
تاريخ النشر: | 01/05/2019 |
موجز عن البحث: | Background: The predominance of specific bacteria such as adherent-invasive Escherichia coli (AIEC) within the
Crohn’s disease (CD) intestine remains poorly understood with little evidence uncovered to support a selective pressure underlying their presence. Intestinal ethanolamine is however readily accessible during periods of intestinal inflammation and enables pathogens to outcompete the host microbiota under such circumstances. Methods: Quantitative RT-PCR (qRT-PCR) to determine expression of genes central to ethanolamine metabolism. transmission electron microscopy to detect presence of bacterial microcompartments (MCPs); in vitro infections of both murine and human macrophage cell lines examining intracellular replication of the AIEC-type strain LF82 and clinical E. coli isolates in the presence of ethanolamine; determination of E. coli ethanolamine utilization (eut) operon transcription in faecal samples from healthy patients, patients with active CD and the same patients in remission following treatment. Results: Growth on the intestinal short chain fatty acid propionic acid (PA) stimulates significantly increased transcription of the eut operon (fold change relative to glucose: N16.9; p-value b.01). Additionally, ethanolamine was accessible to intra-macrophage AIEC and stimulated significant increases in growth intracellularly when it was added extracellularly at concentrations comparable to those in the human intestine. Finally, qRT-PCR indicated that expression of the E. coli eut operon was increased in children with active CD compared to healthy controls (fold change increase: N4.72; P b .02). After clinical remission post-exclusive enteral nutrition treatment, the same CD patients exhibited significantly reduced eut expression (Pre vs Post fold change decrease: N15.64; P b .01). Interpretation: Our data indicates a role for ethanolamine metabolism in selecting for AIEC that are consistently overrepresented in the CD intestine. The increased E. coli metabolism of ethanolamine seen in the intestine during active CD, and its decrease during remission, indicates ethanolamine use may be a key factor in shaping the intestinal microbiome in CD patients, particularly during times of inflammation. |
المؤتمرات العلمية |
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المؤتمر (1): | |
عنوان المؤتمر: | World Microbe Forum |
تاريخ الإنعقاد: | 20/06/2021 |
مكان الإنعقاد: | Virtually |
طبيعة المشاركة: | Poster presentation |
عنوان المشاركة: | Investigation of the impact of food preservatives on avian pathogenic Escherichia coli (APEC) and their role in driving zoonotic disease |
ملخص المشاركة: | The excessive use of antibiotics in agriculture is routinely described as a major contributor to bacterial antimicrobial resistance. Globally, antibiotics are also widely used as growth supplements in livestock. This has led to concerns regarding use of human-use antibiotics in food and food-producing animals. In more recent times organic acids such as propionic acid (PA) and formic acid (FA)have been used as alternative antimicrobials or preservatives in place of antibiotics. PA is a short chain fatty acid naturally abundant in the human and animal intestine as a breakdown product of non-digestible carbohydrates. In the human intestine it plays important roles in regulating the immune response in the human body. Recently, a study has shown that exposure of a Crohn’s Disease (CD) associated bacterial pathotype, adherent-invasive Escherichia coli (AIEC), to PA significantly altered its phenotype resulting in increased adhesion and invasion of epithelial cells and increased persistence through biofilm formation. AIEC are both evolutionarily and phylogenetically related to avian pathogenic Escherichia coli (APEC). Our results indicate that virulence of some APEC strains is increased by exposure to alternative antimicrobials such as PA and FA. This included increased adhesion of APEC strain E. coli 601 to human intestinal epithelial cells after exposure to FA which could be a potential risk of zoonotic disease. Further investigation of these APEC strains is currently underway in a fermentation model of the poultry gut. This approach will improve our understanding of how commonly used preservatives alter the gut microbiota composition and APEC. |
المرفقات
- https://uksacb.org/wp-content/uploads/Abstract.pdf
- https://uksacb.org/wp-content/uploads/Certificate_of_presentation-.pdf
- https://uksacb.org/wp-content/uploads/Confirmation-of-Conference-registration_1.pdf
- https://uksacb.org/wp-content/uploads/Confirmation-of-Conference-registration_2.pdf
- https://uksacb.org/wp-content/uploads/Research-Paper_Adherent-Invasive-Escherichia-coli.pdf