|مجال التميز||تميز دراسي و بحثي|
|عنوان البحث:||Impact of Prenatal and Postnatal Treatment of Sodium Fluoride and Aluminum Chloride on Some Hormonal and Sensorimotor Aspects in Rats|
|رابط إلى البحث:||Click here|
|موجز عن البحث:||In most communities, there is a constant exposure to environmental pollutants with probable negative impact on the development of the nervous system. Among these pollutants are the sodium fluoride (NaF) and aluminum chloride (AlCl3) which may represent a real threat to the proper functioning of the brain. This study comprises two fundamentally different strategies; in the first one, pregnant rats were administered a daily dose of NaF (0.15 g /L) or AlCl3 (500 mg/L) in the drinking water either separately or in combination with each other from day 6 of gestation until just after weaning. In the second approach, the male rats born to mothers exposed to the pollutants were divided into two groups. In the first, rats were continued to be treated with the same pollutants administered to them in the drinking water at the same dose level until the age of 70 days. The rats of the second group were supplied with drinking water without either one of the pollutants for a similar period of time. The rats exposed to NaF separately or in combination with AlCl3 during the prenatal life and subsequently through the postnatal stages exhibited disturbance in the locomotor activities. This was concomitant with alterations in plasma, PTH, ACTH, and estradiol levels. Additionally, the serum levels of LH and testosterone were altered in the two groups treated with sodium fluoride during the prenatal and up to the weaning periods or in the group which continued to have the NaF until day 70 after birth.|
Impacts of feeding fermented soybean on gut morphology and microbes in broiler chicks
|رابط إلى البحث:||Click here|
|موجز عن البحث:||The present examination intended to research the impacts of fermented soya bean application in broiler diet on the creation of the gastrointestinal microbes and some gut histomorphological parameters. Eighty commercial Ross x Ross male broiler chicks were utilized and arbitrarily designated into 2 dietary groups; soyabean – based deit (SBM-deit) and fermented Soyabean based deit (FSBM-deit). The control birds were sustained a corn-SBM based eating regimen, and the treatment bolstered with a cornFSBM based eating routine (1.5 %). Two nourishing stages were utilized, starter (0 to 21 d) and grower (21 to 49 d) stages. Toward the finish of the period (49d), samples of intestinal content (crop, proventriculus + gizzard, ileum and caeca) were gotten and immediately utilized for assurance of digesta pH and developing microorganisms utilizing specific media including the aggregate anaerobic microbes, coliform organisms, streptococci and lactobacilli . Different intestinal tissue samples were likewise collected for histomorphological studies including villus height and crypt depth .In all (gastrointestinal compartments, the arrangement of gut microbes were observed to be changed reliably. The quantity of coliforms were altogether diminished and the quantity of Lactobacilli were fundamentally expanded, in crop, proventriculus + gizzard and ileum. In the caeca, the quantity of Streptococci were altogether brought down. Feeding FSBM enhanced the stomach acid barrier. Both villi at the mid-jejunum and mid-ileum were higher when contrasted with SBM-bolstered chicken. Nonetheless, no significant contrasts were found in crypt depth for all cases considered. Conclusively, FSBM-diet may improve feed to gain ratio and gut health in broilers. All the more particularly, the villus height of small intestinal tissue was more prominent and the organization of the microorganisms inside the gastro-digestive system was valuably moved.|
|عنوان المؤتمر:||Integrative Proteomics: Protein Function, Cellular Systems and Big Data conference|
|مكان الإنعقاد:||Southampton, UK|
|طبيعة المشاركة:||Poster presentation|
|عنوان المشاركة:||Identifying the interaction partners and substrates of oncogenic deubiquitinating enzyme USP7 in colorectal cancer|
The ubiquitin-specific protease USP7 is a deubiquitinase that interacts and targets various substrate proteins. USP7 stabilizes many substrates by the deubiquitination process that preserves them from proteasomal degradation and is an important regulator of cell homeostasis and survival. There is also significant interest in therapeutically targeting USP7 and defining its substrates and interaction partners is an important goal. Our goal is to define the USP7 associated protein network and understand USP7’s role in the regulation of oncogenic proteins in colorectal cancer.
As a cell-model for proteomic analysis, we are using LS174T cell expressing inducible siRNA to knock-down expression of USP7 (Kessler et al., 2007)1 .Depletion of USP7 significantly decreases the viable cells and cell growth in this model and also decreases the abundance of known interaction partners such as DNA Methyltransferase I (Dnmt1). We are using quantitative LC-MS/MS in conjunction with this inducible knock-down and in this presentation will present preliminary results from this study. Our findings may offer a new approach for the determination of the molecular effects of USP7 on DNMT1 and cell survival. This may open up new horizons for targeting USP7 as new molecular targets for the treatment of colorectal cancer.