|مجال التميز||تميز دراسي وبحثي|
Biomodification of a Class-V Restorative Material by Incorporation of Bioactive Agents
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Restoring subgingival class-V cavities successfully, demand special biological properties from a restorative material. This study aimed to assess the effects of incorporating bioactive materials to glass ionomer cement (GIC) on its mechanical and biological properties. Hydroxyapatite, chitosan, chondroitin sulphate, bioglass, gelatine and processed bovine dentin were incorporated into a GIC restorative material. Compressive strength, biaxial flexural strength (BFS), hardness, setting and working time measurements were investigated. Biocompatibility of the new materials was assessed using both monolayer cell cultures of normal oral fibroblasts (NOF) and TR146 keratinocytes, and a 3D-tissue engineered human oral mucosa model (3D-OMM) using presto-blue tissue viability assay and histological examination. Significant reduction in the compressive strength and BFS of gelatine-modified discs was observed, while chondroitin sulphate-modified discs had reduced BFS only (p value > 0.05). For hardness, working and setting times, only bioglass caused significant increase in the working time. NOF viability was significantly increased when exposed to GIC-modified with bovine dentine, bioglass and chitosan. Histological examination showed curling and growth of the epithelial layer toward the disc space, except for the GIC modified with gelatine. This study has highlighted the potential for clinical application of the modified GICs with hydroxyapatite, chitosan, bioglass and bovine dentine in subgingival class-V restorations.
2018 IADR/PER General Session
Biomodification of Class-V Restorative Material by Incorporation of Bioactive Agents
Objectives: Glass ionomer cement (GIC) is the material of choice for restoring class-V cavities. However, there is a need for an improved restorative material to enable periodontal tissue attachment onto the surface of subgingival class-V restorations. Therefore, the aim of this study was biomodification of a GIC-based restorative system by incorporating bioactive materials to enhance soft tissue attachment
Methods: Six bioactive materials including Hydroxyapatite, Chitosan, Chondroitin Sulphate, Bio-glass, Gelatine and Processed Bovine Dentin were incorporated into a conventional GIC restorative material. Ten discs were prepared from each material to measure compressive strength, biaxial flexural strength and hardness. For setting and working time five discs were prepared. Biocompatibility was assessed using Presto-blue (PB) assay on normal oral fibroblasts (NOF), TR146 keratinocytes and 3D tissue engineered oral mucosa models (OMM) exposed to the test materials.
Results: Statistical analysis showed that the compressive strength of the biomodified restorative materials were comparable to the conventional GIC except for gelatine and chondroitin sulphate groups. For flexural strength, only gelatine group showed significantly lower values. For hardness and setting time, no statistically significant difference was observed between the groups except for bio-glass which showed significantly higher working time, compared to the conventional GIC. Biocompatibility testing using NOF monolayer cell culture systems and OMM showed processed bovine dentin and bio-glass groups had significantly higher cell viability in comparison with the conventional GIC.
Conclusions: The addition of processed bovine dentin and bio-glass to the conventional GIC enhances the material’s bioactivity without compromising its mechanical properties and therefore may have a potential clinical application in subgingival class-V restorations to enhance periodontal soft tissue attachment.
حصلت الطالبة على الزمالة الملكية البريطانية
(The student successfully passed the Membership in Prosthodontics (M Prost RCSEd) of the College on 04-Sep-2020 and she was elected a Member at the meeting of the Council of the College held on 15-Nov-2020.)